Response of Cupressus funebris Tissue Cultures to Gibberellins

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Science  17 Jun 1960:
Vol. 131, Issue 3416, pp. 1806-1807
DOI: 10.1126/science.131.3416.1806


Gibberellins are a specific growth requirement of tissue cultures derived from the staminate cones of Cupressus funebris. The tissue originally required coconut milk for growth. However, a mixture of acid-hydrolyzed casein hydrolyzate and gibberellins replaces the coconut milk entirely and permits even better growth. It is tentatively suggested that coconut milk contains gibberellins or related substances and that these substances are involved in the nitrogen metabolism of the tissue.