Use of Pectinase in Preparation of Mitochondria from Tobacco-Tissue Cultures

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Science  09 Dec 1960:
Vol. 132, Issue 3441, pp. 1766-1767
DOI: 10.1126/science.132.3441.1766


Mitochondrial particles were isolated from tobacco cells in tissue cultures which were disintegrated with pectinase. The pectinase treatment of the tissue cultures reduced the dry weight of the particulate preparations and eliminated glutinous material, but caused little change in oxidative and phosphorylative activities. The pectinase apparently did not affect the enzymatically active sites of the particles.