Blockade of Deoxyribonucleic Acid Synthesis by Deuterium Oxide

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Science  14 Apr 1961:
Vol. 133, Issue 3459, pp. 1131-1133
DOI: 10.1126/science.133.3459.1131


Interference with deoxyribonucleic acid replication need not be a primary mechanism in the blockade of cell division by deuterium oxide, but current hypotheses on the molecular basis of the blockade do suggest that such interference might take place under appropriate conditions. Autoradiographic experiments support the suggestion, for whereas normal sea urchin eggs incorporate H3-thymidine into deoxyribonucleic acid from almost the beginning of development, cells immersed in deuterium-enriched media do not. Blockade of mitosis and inhibition of thymidine incorporation are simultaneously relieved when the eggs are returned to normal water.