Axenic Cultivation of Entamoeba histolytica

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Science  04 Aug 1961:
Vol. 134, Issue 3475, pp. 336-337
DOI: 10.1126/science.134.3475.336


Entamoeba histolytica (National Institutes of Health strain 200) has been maintained in axenic culture through 50 transfers over a period of 6 months. The medium used is diphasic and contains a cell-free extract of chick embryo which is essential for growth. Inocula of 50,000 amoebas yield 150,000 to 200,000 organisms in 72 hours of incubation at 35°C. An extract prepared from boiled embryos supports growth equally well.

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