Effect of Enzymes on Partially Purified Japanese B Encephalitis and Related Arbor Viruses

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Science  08 Dec 1961:
Vol. 134, Issue 3493, pp. 1878-1880
DOI: 10.1126/science.134.3493.1878-a


Japanese B encephalitis and some other arbor viruses were partially purified by cellulose column chromatography or by fluorocarbon deproteinization and tested for sensitivity to enzymes. Infectivity decreased markedly when the viruses were mixed with trypsin or pancreatic lipase at 37°C. The enzymes also impair the immunogenicity of the virus in rabbits. Poliovirus is resistant to the enzymes.

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