Catalysis by Imidazole of Deuterium-Hydrogen Exchange in Amide N-H Groups

See allHide authors and affiliations

Science  16 Nov 1962:
Vol. 138, Issue 3542, pp. 830-831
DOI: 10.1126/science.138.3542.830


Imidazole, at concentrations of 0.05M to 0.2M, has been found to increase the rate of deuterium-hydrogen exchange in a model amide by 40-fold or more. Since imidazole side-chains occur in proteins, deuterium exchange in these macromolecules may be strongly affected by the presence of these or similar activating groups.