Bohr Effect: Absence in a Molluscan Hemocyanin

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Science  29 Mar 1963:
Vol. 139, Issue 3561, pp. 1294-1295
DOI: 10.1126/science.139.3561.1294


The hemocyanin of the keyhole limpet, Diodora aspera, shows no Bohr effect within the pH range 6.88 to 7.84. At 10°C the pressure of half-saturation is 5 mm-Hg of oxygen pressure. A moderately positive interaction occurs among the oxygen-combining sites during oxygenation. The heat of oxygenation is calculated to be approximately -12.6 kcal/mole of oxygen. The pH of normal blood of Diodora is lower than that of many other marine molluscs.