Immune Response and Mitosis of Human Peripheral Blood Lymphocytes in vitro

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Science  29 Nov 1963:
Vol. 142, Issue 3596, pp. 1185-1187
DOI: 10.1126/science.142.3596.1185


Phytohemagglutinin causes cultured lymphocytes to agglutinate, divide, and produce γ-globulin. Most cells are transformed into large lymphocytes, some resembling plasmocytes. Actinomycin D stops γ-globulin production after 2 hours. When specific antigens are added to lymphocytes from sensitized individuals, only some cells undergo morphological transformation, produce γ-globulin, and divide. When cells or cell extracts from an unrelated individual are added to a culture, a similar reaction occurs.