N-Ethylmaleimide Inhibition of Horseshoe Crab Hemocyte Agglutination

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Science  29 May 1964:
Vol. 144, Issue 3622, pp. 1147-1148
DOI: 10.1126/science.144.3622.1147


The sulfhydryl inhibitor N-ethylmaleimide (NEM) inhibits the agglutination of horseshoe crab hemocytes. The inhibitory action is neutralized by adding cysteine to the NEM before it is mixed with the hemolymph. This new method for arresting horseshoe crab hemocyte agglutination suggests the participation of sulfhydryl groups in the process.

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