Fluorescence Polarization: Measurement with Ultraviolet-Polarizing Filters in a Spectrophotofluorometer

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Science  12 Feb 1965:
Vol. 147, Issue 3659, pp. 729-732
DOI: 10.1126/science.147.3659.729


Ultraviolet-polarizing filters with large apertures facilitate the use of spectrophotofluorometers for measuring fluorescence polarization. Such filters (i) allow the use of polarized exciting light even in the ultraviolet region, (ii) make it possible to correct for grating anomalies which influence the observed polarization, and (iii) because of good transmission properties allow the entrance of sufficient light so that small slits and dilute solutions can be used. Under optimum conditions, polarization can be measured with a precision of ±0.002. Results obtained are in excellent agreement with values cited elsewhere and indicate that standard spectrophotofluorometers can easily be adapted for accurate measurement of polarization.

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