Tetracycline: Effect on Osteogenesis in vitro

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Science  20 Aug 1965:
Vol. 149, Issue 3686, pp. 870-872
DOI: 10.1126/science.149.3686.870


Tetracycline hydrochloride added to cultures of rudiments of embryonic bone in a concentration of 1 microgram per milliliter prevents mineralization of the bones and is found throughout the calcified zones. The inhibition is reversible; upon transfer of a treated culture to a normal medium, mineralization proceeds normally but maldeveloped bones result.