Inhibitory Oxidation Products of Indole-3-Acetic Acid: Enzymic Formation and Detoxification by Pea Seedlings

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Science  10 Sep 1965:
Vol. 149, Issue 3689, pp. 1249-1251
DOI: 10.1126/science.149.3689.1249


Extracts of etiolated pea seedlings oxidize indole-3-acetic acid, a plant auxin, to 3-hydroxymethyloxindole. At physiological pH this compound is dehydrated to 3-methyleneoxindole, a highly reactive sulfhydryl reagent and inhibitor of cell growth. 3-Methlyeneoxindole is in turn detoxified by an enzymatic, triphosphopyridine nucleotide-linked reduction to 3-methyloxindole, a nontoxic compound. These enzymatic conversions may be responsible for some of the responses to auxin, particularly sensitivity to its inhibitory effects on growth.

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