Preservation of Mammalian Cells in a Chemically Defined Medium and Dimethylsulfoxide

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Science  10 Sep 1965:
Vol. 149, Issue 3689, pp. 1266-1267
DOI: 10.1126/science.149.3689.1266


Three established mammalian cell lines (cat kidney, L, and HeLa cells), grown in suspension in a protein-free, chemically defined medium, were stored under liquid nitrogen in the defined medium fortified with 4 and 8 percent dimethylsulfoxide. After storage for 1 month the recovery viability was 86 percent, and cells showed normal growth upon reinoculation into the defined growth medium.

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