Differentiation of Tobacco Plants from Single, Isolated Cells in Microcultures

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Science  12 Nov 1965:
Vol. 150, Issue 3698, pp. 889-892
DOI: 10.1126/science.150.3698.889


Single cells isolated from pith callus of fresh stem of hybrid tobacco (Nicotiana glutinosa ♂ x N. tabacum ♀) gave rise to small colonies of cells in microcultures which upon transfer to agar medium produced clones of callus tissue. These single-cell clones differentiated roots, and shoots with green leaves, on a completely defined nutrient medium. The rooted shoots developed into normal plants after transfer to soil in pots in the greenhouse. Buds and flowers were produced by these plants.