Gene-Specific Messenger RNA: Isolation by the Deletion Method

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Science  21 Jan 1966:
Vol. 151, Issue 3708, pp. 328-330
DOI: 10.1126/science.151.3708.328


Messenger RNA molecules, homologous to a small portion of the genome of bacteriophage T4, have been isolated. RNA fragments specific to the rII A and the rII B cistrons have been separated by hybridization with DNA isolated from appropriate deletion mutants. An RNA species homologous in nucleotide sequence to a defined part of the rII A cistron has been identified.