Lymphocystis Virus: Isolation and Propagation in Centrarchid Fish Cell Lines

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Science  25 Feb 1966:
Vol. 151, Issue 3713, pp. 1004-1005
DOI: 10.1126/science.151.3713.1004


A virus from fish with lymphocystis disease was isolated in fish cell cultures. Eleven serial transfers were made and the pathognomonic lymphocystis cells were produced in vitro in each transfer. Fish inoculated with 6th- and 9th-passage material developed the disease, and virus was reisolated front them.