Autoradiography with Tritiated Methotrexate and the Cellular Distribution of Folate Reductase

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Science  25 Mar 1966:
Vol. 151, Issue 3717, pp. 1528-1530
DOI: 10.1126/science.151.3717.1528


Bound methotrexate has been revealed by an autoradiographic procedure, presumed to introduce a method for cytochemical study of folate reductase. Preferential localization is seen in kidney proximal tubules, intestinal epithelium, and nuclei of parenchymal liver cells in mice. The extremely firm binding and prolonged retention of this drug should render it suitable as an inert label for the autoradiographic study of cell migrations and lifetimes.

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