End-Organ Effects of Thyroid Hormones: Subcellular Interactions in Cultured Cells

See allHide authors and affiliations

Science  12 Aug 1966:
Vol. 153, Issue 3737, pp. 763-765
DOI: 10.1126/science.153.3737.763


Both actinomycin D and puromycin suppress the formation of colonies by cultured human kidney epithelial cells (T-l), but inactivation by puromycin is partially reversed with thyroid hormones. Uptake by the cells of L-thyroxine labeled with iodine-125, 60 to 80 percent of which is nuclear, is depressed by actinomycin and enhanced by puromycin. Genome and possibly nuclear membrane are implicated as initiating loci.