Polysomes Extracted from Escherichia coli by Freeze-Thaw-Lysozyme Lysis

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Science  02 Sep 1966:
Vol. 153, Issue 3740, pp. 1119-1120
DOI: 10.1126/science.153.3740.1119


Polysomes can be extracted from Escherichia coli by freezing and thawing in the presence of lysozyme, followed by treatment with sodium deoxycholate. The method is simple and convenient; the yields consistently high.