Electrophoretic Variation of Galactose-1-Phosphate Uridyltransferase

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Science  02 Dec 1966:
Vol. 154, Issue 3753, pp. 1179-1180
DOI: 10.1126/science.154.3753.1179


A specific method for starch-gel electrophoresis of galactose-1-phosphate uridyltransferase has been developed. Electrophoresis of red-cell hemolyzate from normal subjects and subjects homozygous for the Duarte variant has shown that the Duarte variant has a slightly faster electrophoretic mobility than the normal enzyme under the various conditions used. Molecular-weight estimation on Sephadex G-200 indicates that this observed difference in electrophoreticmobility of the Duarte variant is not due to difference in molecular size. Both enzymes have a molecular weigh of approximately 85,000.