Establishment of Four Functional, Clonal Strains of Animal Cells in Culture

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Science  02 Dec 1966:
Vol. 154, Issue 3753, pp. 1186-1189
DOI: 10.1126/science.154.3753.1186


The single-cell plating technique was used to develop four clonal cell lines that perform organ-specific functions after being serially cultured for prolonged periods. These strains include steroid-secreting Leydig cells, melanomacells that form pigment, and two strains from a hormone-secreting rat pituitary tumor. One of the cell lines from the pituitary tumor secretes growt hormone, while another line derived from the same tumor secretes a substance similar to adrenocorticotropic hormone.