Collagen-Coated Cellulose Sponge: Three-Dimensional Matrix for Tissue Culture of Walker Tumor 256

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Science  10 Mar 1967:
Vol. 155, Issue 3767, pp. 1259-1261
DOI: 10.1126/science.155.3767.1259-a


Three-dimensional growth of large populations of cells in vitro has been observed in the interstices of a matrix consisting of collagen-coated cellu lose sponge. The growth of Walker tumor 256 in this composite matrix is com pared with that found in a matrix composed of either cellulose sponge alone or collagen sponge alone. The composite matrix is superior to either one. Collagen coated cellulose sponge may provide a simple tool for the study of social interaction of cells in the formation of organized elementary tissue structures.