D-Glucose: Preferential Binding to Brush Borders Disrupted with Tris(hydroxymethyl) aminomethane

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Science  10 Mar 1967:
Vol. 155, Issue 3767, pp. 1261-1263
DOI: 10.1126/science.155.3767.1261


The actively transported sugar D-glucose binds to brush borders disrupted with tris(hydroxymethyl)aminomethane in preference to D-mannose and L-glucose, which are not actively transported. This preferential binding of D-glucose is not dependent on either added Na+ or adenosine triphosphatase activity stimulated by Na+ with K+ and Mg2+, but it is temperature-dependent and is completely inhibited by 0.1 millimolar phlorizin and 1 millimolar mercuric chloride.