Prevention of Protein Denaturation during Exposure to Sterilization Temperatures

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Science  10 Mar 1967:
Vol. 155, Issue 3767, pp. 1287-1288
DOI: 10.1126/science.155.3767.1287


Firefly luciferase exposed to a temperature of 135°C for 36 hours re tained up to 40 percent of its original activity. Prerequisites for heat stability were the use of a molecular sieve (Seph adex G-25 or Biogel P-300) and a high vacuum (5 x 10-4 mm-Hg). These studies present a possible solution to the problem of sterilization for exobiologi cal experiments.