Tetanus Toxin: Fine Structure Localization of Binding Sites in Striated Muscle

See allHide authors and affiliations

Science  09 Feb 1968:
Vol. 159, Issue 3815, pp. 643-644
DOI: 10.1126/science.159.3815.643


When striated muscle from mice poisoned with purified tetanus neurotoxin was incubated with tetanus antitoxin labeled with horseradish peroxidase and stained for peroxidase activity, an electron-opaque reaction product was found in the transverse and terminal sacs of the longitudinal elements of the sarcotubular system. Demonstration of toxin in this location suggests that it acts by interfering with contraction coupling or mechanisms of contraction-relaxation.