Enzyme-Catalyzed Reactions of the Carcinogen N-Hydroxy-2-fluorenylacetamide with Nucleic Acid

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Science  22 Mar 1968:
Vol. 159, Issue 3821, pp. 1351-1353
DOI: 10.1126/science.159.3821.1351


A protein fraction obtained by gel filtration of a 105,000g supernatant of rat liver catalyzes three reactions of the hepatocarcinogen N-hydroxy-2-fluorenylacetamide with nucleic acid. Cofactor requirements and isotopic studies suggest that the reactive intermediates involved may be N-2-fluorenylhydroxylamine, and phosphate and sulfate esters of N-hydroxy-2-fluorenylacetamide.

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