Plantlets from Aspen Tissue Cultures

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Science  14 Jun 1968:
Vol. 160, Issue 3833, pp. 1234-1235
DOI: 10.1126/science.160.3833.1234


After 2 weeks in the dark, leafy shoots were initiated on subcultured callus tissue of triploid quaking aspen on Wolter's medium without auxin and with 6-benzylaminopurine at 0.05, 0.10., 0.15, or 0.20 milligram per liter substituted for kinetin. Shoots transferred to Wolter's medium under light (323 milliphots) grew large roots and were isolated as plantlets.