Differentiated Rat Glial Cell Strain in Tissue Culture

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Science  26 Jul 1968:
Vol. 161, Issue 3839, pp. 370-371
DOI: 10.1126/science.161.3839.370


Rat glial tumors, induced by injections of N-nitrosomethylurea, were plated and propagated in culture. Among a few cell strains obtained, one clone contains S-100 protein, which is unique to brain in vertebrates. Stationary-phase cultures contain approximately ten times more S-100 protein per cell than exponentially growing cells. When injected into newborn rats, cells producing S-100 grew as a glial tumor, which contained S-100 protein.

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