RNA-DNA Hybridization: Demonstration in a Mammalian System of Competition by Preincubation

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Science  09 Aug 1968:
Vol. 161, Issue 3841, pp. 570
DOI: 10.1126/science.161.3841.570


Nonspecific intermolecular interactions obscure the interpretation of competition experiments involving hybridization of DNA and RNA in mammalian systems when the labeled RNA/DNA ratio is low. The effect of these interactions can be minimized by choosing a sufficiently high ratio of labeled RNA/DNA. No serious effect of nonspecific interactions is observed in bacteriophage systems, even at very low labeled RNA/DNA ratios.

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