Cytokinin from Soluble RNA of Escherichia coli: 6-(3-Methyl-2-butenylamino)-2-methylthio-9-β-D-ribofuranosylpurine

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Science  16 Aug 1968:
Vol. 161, Issue 3842, pp. 691-693
DOI: 10.1126/science.161.3842.691


We have isolated a compound responsible for the cytokinin activity of soluble RNA from Escherichia coli. The structure, indicated as 6-(3-methyl-2-butenylamino)-2-methylthio-9-β-D-ribofuranosylpurine, C16H23N504S, on the basis of low-and high-reso!ution mass spectrometry, was established by unequivocal synthesis. The mass spectra, chromatographic behavior, and ultraviolet spectra of the compounds from natural and synthetic sources were identical.

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