Hydrolysis at Arginylproline in Polypeptides by Clostridiopeptidase B

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Science  18 Oct 1968:
Vol. 162, Issue 3851, pp. 374-375
DOI: 10.1126/science.162.3851.374


Clostridiopeptidase B, a sulfhydryl protease from Clostridium histolyticum, hydrolyzes the arginylproline bond in the synthetic polypeptide methionyllysyl-bradykinin. Hydrolysis also occurs at a reduced rate at the lysylarginine bond, further delineating the environment necessary for the minor proteolysis seen with this enzyme at lysine residues. The specificity of clostridiopeptidase B differs from trypsin, which hydrolyzes this synthetic polypeptide only at the lysylarginine bond.