Nonspecific Staining: Its Control in Immunofluorescence Examination of Soil

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Science  29 Nov 1968:
Vol. 162, Issue 3857, pp. 1012-1014
DOI: 10.1126/science.162.3857.1012


Gelatin preparations were used to treat soil slides prior to addition of fluorescent antibody. Nonspecific staining was avoided, with no detectable interference to specific staining. Gelatin-rhodamine conjugates served to counterstain as well as to prevent nonspecific staining.

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