Single Crystals of Transfer RNA from Formylmethionine and Phenylalanine Transfer RNA's

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Science  20 Dec 1968:
Vol. 162, Issue 3860, pp. 1384-1387
DOI: 10.1126/science.162.3860.1384


Reproducible conditions have been developed for crystallization of transfer RNA. The conditions may be applicable to many pure transfer RNA species since identical procedures (except for initial transfer-RNA concentration) yielded good crystals from both yeast and Escherichia coli transfer RNA. These crystals, which must be kept at temperatures below about 10°C and handled in vapor of controlled alcohol concentration, have been studied by x-ray crystallography. The availability of crystals of a nucleic acid opens a route for extending knowledge of the tertiary structure of transfer RNA and its relation to important biological functions.

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