Cytoplasmic DNA from Petite Colonies of Saccharomyces cerevisiae: A Hypothesis on the Nature of the Mutation

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Science  21 Mar 1969:
Vol. 163, Issue 3873, pp. 1331-1333
DOI: 10.1126/science.163.3873.1331


The density of the cytoplasmic DNA of two strains of "petite" mutants of yeast, obtained by treatment with acriflavin and with ultraviolet light, was examined in cesium chloride density-gradient centrifugation and in all cases appeared to be less than that of the wild type. A cytoplasmic respiratory-deficient strain, treated with additional acriflavin, can show a further shift of the position of the satellite band, always in the direction of reduction of density. Also, from the p+ x p- cross, p- strains can be recovered in which the density of the satellite DNA is different from the density of the parent p- strain. This finding suggests the existence of recombination in cytoplasmic DNA moleciules.

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