Acetylcholine Receptor: Covalent Attachment of Depolarizing Groups at the Active Site

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Science  20 Jun 1969:
Vol. 164, Issue 3886, pp. 1420-1421
DOI: 10.1126/science.164.3886.1420


Following reduction of the acetylcholine receptor in the electroplax with dithiothreitol, the quaternary ammonium compounds bromoacetylcholine bromide and the p-nitrophenyl ester of (p-carboxyphenyl) trimethylammonium iodide react near the active site probably with a sulfhydryl group. The covalently attached quaternary ammonium moieties additionally interact with the active site noncovalently to activate the receptor and cause depolarization of the cell.

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