Chromatin and Histones: Binding of Tritiated Actinomycin D to Heterochromatin in Mealy Bugs

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Science  27 Jun 1969:
Vol. 164, Issue 3887, pp. 1527-1529
DOI: 10.1126/science.164.3887.1527


The degree of actinomycin D binding to DNA in chromatin is dependent upon the state of repression of chromatin. Living cells bind three times more tritiated actinomycin to euchromatin than to genetically inactive heterochromatin. Extraction of histone results in a general increase in tritiated actinomycin binding and in a ratio of the uptake in heterochromatin to that in euchromatin approaching unity.

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