25-Hydroxycholecalciferol: Direct Effect on Calcium Transport

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Science  25 Jul 1969:
Vol. 165, Issue 3891, pp. 405-407
DOI: 10.1126/science.165.3891.405


The perfused small intestine from a vitamin D deficient rat exhibits about one-half the calcium transport of the intestine from a rat given vitamin D. These levels of calcium transport can be maintained for at least 4 hours. Addition of 2.5 micrograms of 25-hydroxycholecalciferol added to the vitamin D deficient intestine via the arterial blood perfusate induces a rise in calcium transport to +D levels within 2 hours. In contrast, 250 micrograms of vitamin D3 given in the same manner has no effect on the calcium transport level overa 4-hour period. These data provide strong evidence that 25-hydroxycholecalci ferol represents the metabolically active form of vitamin D3.

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