Parasexual Cycle in Cultivated Human Somatic Cells

See allHide authors and affiliations

Science  07 Nov 1969:
Vol. 166, Issue 3906, pp. 761-763
DOI: 10.1126/science.166.3906.761


Somatic segregation for three different autosomes was demonstrated in two strains of human diploid fibroblasts derived from subjects known to be heterozygous for chromosomal variants. Recombinant diploid cells appeared within cultures of tetraploid clones isolted from mass cultures. Tetraploid cells regularly occur in mass cultures and within clones of diploid cells. Such a parasexual cycle (2n→4n→2n), with recombination of entire linkage groups, could from the basis of a beginning formal genetic analysis in man.

Stay Connected to Science