Absorption of Proteins and Peptides in the Far Ultraviolet

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Science  09 Jan 1970:
Vol. 167, Issue 3915, pp. 179-181
DOI: 10.1126/science.167.3915.179


An absorption peak for the peptide bond at 187 nanometers has been confirmed; a protein assay at this wavelength allows quantitation of proteins in aqueous solution at concentrations between 0.1 and 25 micrograms per milliliter. Assays are conducted at 6°C to take advantage of the reduction of end-absorption of water with temperature.

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