Countercurrent Chromatography: Liquid-Liquid Partition Chromatography without Solid Support

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Science  16 Jan 1970:
Vol. 167, Issue 3916, pp. 281-283
DOI: 10.1126/science.167.3916.281


The liquid-liquid partition chromatographic system reported here involves a long helix of narrow-bore tubing. When the coiled tube is filled with one phase of a two-phase system and fed with the other phase, phase-interchange takes place in each turn of the coil, leaving a segment of the former phase as the stationary phase. Consequently, solutes present in either phase are subjected to a multistep partition process. The column efficiency, estimated on a separation of dinitrophenyl amino acids, is comparable to that of gas chromatography.

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