Isolation and Zonal Fractionation of Metaphase Chromosomes from Human Diploid Cells

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Science  20 Feb 1970:
Vol. 167, Issue 3921, pp. 1141-1143
DOI: 10.1126/science.167.3921.1141-a


A simple method for isolating large quantities of human metaphase chromosomes has been developed. Fractionation of chromosomes from peripheral lymphocytes on a large scale is accomplished by velocity sedimentation in an A-XII zonal rotor.

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