Spontaneous Rhythmic Contraction of Separated Heart Muscle Cells

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Science  27 Mar 1970:
Vol. 167, Issue 3926, pp. 1727-1729
DOI: 10.1126/science.167.3926.1727


Muscle cells that conitract spontaneously and rhythmically can be obtained from adult mouse myocardium. Contractions are observed immediately after homogenization in a solution that is ionically similar to intracellular fluid. Contraction frequency varies directly with temperature and decreases as a function of time after homogenization. At 16°C rhythmic relaxation and contraction occur for about 20 minutes. Contractions are dependent on the presence of adenosine triphosphate in the homogenization medium.

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