Inside-Out Red Cell Membrane Vesicles: Preparation and Puirification

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Science  10 Apr 1970:
Vol. 168, Issue 3928, pp. 255-257
DOI: 10.1126/science.168.3928.255


Plasma membranes purified from human red cells were Converted into small vesicleS by disruption in alkaline buffer of low ionic strength. Most of these vesicles were inside-out. The presence of divalent cations prevented this inversion. The inside-out vesicles were separatcd from right-side-out vesicles by centrifugration to equilibrium in dextran density gradients.

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