Colcemid Sensitivity of Fission Yeast and the Isolation of Colcemid-Resistant Mutants

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Science  24 Apr 1970:
Vol. 168, Issue 3930, pp. 485-487
DOI: 10.1126/science.168.3930.485


Cell division of the fission yeast, Schizosaccharomyces pombe, is reversibly inhibited by the antimitotic agent Colcemid (N-deacetyl-N-methylcolchicine) in nutrient medium. Cell growth continiues until all cells become nonseparating cell doublets in a V configuration. Mutants have been isolated capable of uninhibited growth in the presence of concentrations of Colcemid mycostatic for the parent strain.

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