Solid-Phase Radioimmunoassay of Protein Biosynthesis

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Science  19 Jun 1970:
Vol. 168, Issue 3938, pp. 1467-1468
DOI: 10.1126/science.168.3938.1467


Solid-phase radioimmunoassay was used for measuring the rate of radioisotope incorporation into a single protein species. Radioactive antigen was measured by its binding to specific antiserum covalently linked to insoluble bromoacetylcellulose. The insoluble antigen-antibody complex was collected on filters for counting. The assay is performed in about 4 hours as compared with several days for methods based on the precipitin reaction.

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