Double-Helical Polynucleotides: Immunochemical Recognition of Differing Conformations

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Science  07 Aug 1970:
Vol. 169, Issue 3945, pp. 609-611
DOI: 10.1126/science.169.3945.609


Rabbit antibodies to double-helical RNA react by complement fixation with synthetic or natural double-strand RNA but not with native DNA. In turn, human (from systemic lupus erythematosus patients) antibodies to native DNA do not react with double-strand RNA. Both types of antibodies show cross-reactions (from 1 percent to 50 percent) with RNA-DNA hybrids, but antibodies to the hybrids do not react at all with double-strand RNA or with native DNA. Antibodies to polydeoxyguanylate·polydeoxycytidylate also failed to react with native DNA.

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