Desmosterol as the Major Sterol in L-Cell Mouse Fibroblasts Grown in Sterol-Free Culture Medium

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Science  28 Aug 1970:
Vol. 169, Issue 3948, pp. 880-882
DOI: 10.1126/science.169.3948.880


The principal sterol synthesized by L-cell mouse fibroblasts is desmosterol. Cholesterol was not detected in these cells when they were grown in a sterol-free culture medium. These findings indicate that, in cells, cholesterol can be replaced by desmosterol. Sterol analyses of six other tissue culture cell lines revealed cholesterol synthesis.