Tyrosine Transaminase Induction by Dexamethasone in a New Rat Liver Cell Line

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Science  20 Nov 1970:
Vol. 170, Issue 3960, pp. 859-861
DOI: 10.1126/science.170.3960.859


A cell line derived from normal, adult rat liver has been established; the cells are similar to hepatocytes, as shown by electron microscopy. The addition of dexamethasone to the culture medium induced a three- to sixfold increase in the specific activity of tyrosine α-ketoglutarate transaminase; this increase was inhibited by the simultaneous addition of cycloheximide or actinomycin D. The latter, when added to cells given prior treatment with dexamethasone, further enhanced the transaminase activity. Contact-inhibited cells showed a lower response to dexamethasone than exponentially growing cells.

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