Fluorescence of the Purine and Pyrimidine Bases of the Nucleic Acids in Neutral Aqueous Solution at 300°K

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Science  19 Feb 1971:
Vol. 171, Issue 3972, pp. 675-677
DOI: 10.1126/science.171.3972.675


Fluorescence of adenine, guanine, cytosine, and uracil at room temperature in neutral aqueous solution has been detected by means of a digital signal accumulation technique. Corrected emission and excitation spectra are presented and compared with low-temperature data. The quantum yields are, respectively, 2.6 x 10-4, 3.0 x 10-4, 0.8 x 10-4, and 0.5 x 10-4 when the bases are excited at their low-energy absorption maxima.

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