Microsomal Hydroxylase Induction in Liver Cell Culture by Phenobarbital, Polycyclic Hydrocarbons, and p,p'-DDT

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Science  09 Apr 1971:
Vol. 172, Issue 3979, pp. 167-169
DOI: 10.1126/science.172.3979.167


Aryl hydrocarbon hydroxylase induction by phenobarbital, polycyclic hydrocarbons, and the insecticide, 2.2-bis(p-chlorophenyl)-1,1,1-trichloroethane (p,p'-DDT), occurs in rat fetal liver cell cultures. The maximum net rate at which the hydroxylase activity accumulates is about the same when phenobarbital, 3-methlcholanthrene, or benz[a]anthracene is in the growth medium at optimum concentrations. An additive effect is obtained when either phenobarbital or p.p'-DDT is present with a polycyclic hydrocarbon in the growth medium, but not when the cells are treated with phenobarbital plus p.p'-DDT or with the combination of two polycyclic hydrocarbons.

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